› 3tcx

X-ray diffraction
3.6Å resolution

Structure of Engineered Single Domain ICAM-1 D1 with High-Affinity aL Integrin I Domain of Native C-Terminal Helix Conformation

Released:
DOI: 10.2210/pdb3tcx/pdb
PDB entry 3tcx coloured by chain, front view.
PDB entry 3tcx coloured by chain, side view.
PDB entry 3tcx coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Kang S, Kim CU, Gu X, Owens RM, van Rijn SJ, Boonyaleepun V, Mao Y, Springer TA, Jin MM

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138633 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Intercellular adhesion molecule 1 Chains: A, C, E, G, I, K, M, O, Q, S, U, W, Y, a
Molecule details ›
Chains: A, C, E, G, I, K, M, O, Q, S, U, W, Y, a
Length: 85 amino acids
Theoretical weight: 9.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05362 (Residues: 29-112; Coverage: 17%)
Gene name: ICAM1
Sequence domains: Intercellular adhesion molecule (ICAM), N-terminal domain
Structure domains: Immunoglobulins
Integrin alpha-L Chains: B, D, F, H, J, L, N, P, R, T, V, X, Z, b
Molecule details ›
Chains: B, D, F, H, J, L, N, P, R, T, V, X, Z, b
Length: 180 amino acids
Theoretical weight: 20.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P20701 (Residues: 154-332; Coverage: 16%)
Gene names: CD11A, ITGAL
Sequence domains: von Willebrand factor type A domain
Structure domains: von Willebrand factor, type A domain

Ligands and Environments

1 bound ligand:
Ligand MG 14 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P212121
Unit cell:
a: 104.041Å b: 166.334Å c: 299.372Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.218 0.234
Expression system: Escherichia coli

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