› 3t5i

X-ray diffraction
2.1Å resolution

Structure of Fully modified farnesylated Rheb Peptide in complex with PDE6D

Released:
DOI: 10.2210/pdb3t5i/pdb
PDB entry 3t5i coloured by chain, front view.
PDB entry 3t5i coloured by chain, side view.
PDB entry 3t5i coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Arl2-GTP and Arl3-GTP regulate a GDI-like transport system for farnesylated cargo.
Ismail SA, Chen YX, Rusinova A, Chandra A, Bierbaum M, Gremer L, Triola G, Waldmann H, Bastiaens PI, Wittinghofer A
Nat Chem Biol 7 942-9 (2011)
PMID: 22002721

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-129174 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 152 amino acids
Theoretical weight: 17.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43924 (Residues: 1-150; Coverage: 100%)
Gene names: PDE6D, PDED
Sequence domains: GMP-PDE, delta subunit
Structure domains: GMP phosphodiesterase, delta subunit
C-terminal Farnesylated Rheb peptide CSQQGKSS(CMT) Chains: Q, R
Molecule details ›
Chains: Q, R
Length: 9 amino acids
Theoretical weight: 942 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand FAR 4 x FAR
1 modified residue:
Ligand CMT 2 x CMT

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 65.75Å b: 70.05Å c: 71.23Å
α: 90° β: 81.55° γ: 90°
R-values:
R R work R free
0.191 0.189 0.243
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

41 review citations

Regulation of small GTPases by GEFs, GAPs, and GDIs.
Cherfils et al. (2013)
40 more

6 mentions without citation

The prenyl-binding protein PrBP/δ: a chaperone participating in intracellular trafficking.
Zhang et al. (2012)
5 more