› 3t31

X-ray diffraction
2.3Å resolution

Crystal structure of sulfide:quinone oxidoreductase from Acidithiobacillus ferrooxidans in complex with decylubiquinone

Released:
DOI: 10.2210/pdb3t31/pdb
PDB entry 3t31 coloured by chain, front view.
PDB entry 3t31 coloured by chain, side view.
PDB entry 3t31 coloured by chain, top view.
Source organism: Acidithiobacillus ferrooxidans ATCC 23270
Primary publication:
Crystal structure of sulfide:quinone oxidoreductase from Acidithiobacillus ferrooxidans: insights into sulfidotrophic respiration and detoxification.
Cherney MM, Zhang Y, Solomonson M, Weiner JH, James MN
J Mol Biol 398 292-305 (2010)
PMID: 20303979

Function and Biology Details

Reaction catalysed: 
n sulfide + n quinone = polysulfide + n quinol
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110497 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfide-quinone reductase Chain: A
Molecule details ›
Chain: A
Length: 437 amino acids
Theoretical weight: 47.86 KDa
Source organism: Acidithiobacillus ferrooxidans ATCC 23270
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B7JBP8 (Residues: 2-434; Coverage: 100%)
Gene name: AFE_1792
Sequence domains: Pyridine nucleotide-disulphide oxidoreductase
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
3 bound ligands:
Ligand LMT 1 x LMT
Ligand DCQ 1 x DCQ
Ligand H2S 1 x H2S
2 modified residues:
Ligand CSS 1 x CSS
Ligand TSY 1 x TSY

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P6222
Unit cell:
a: 150.77Å b: 150.77Å c: 82Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.161 0.159 0.209
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Chemical Biology of H2S Signaling through Persulfidation.
Filipovic et al. (2018)
12 more

5 mentions without citation

Hydrogen Sulfide Oxidation by Sulfide Quinone Oxidoreductase.
Landry et al. (2021)
4 more