› 3t0o

X-ray diffraction
1.59Å resolution

Crystal Structure Analysis of Human RNase T2

Released:
DOI: 10.2210/pdb3t0o/pdb
PDB entry 3t0o coloured by chain, front view.
PDB entry 3t0o coloured by chain, side view.
PDB entry 3t0o coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and activity of the only human RNase T2.
Thorn A, Steinfeld R, Ziegenbein M, Grapp M, Hsiao HH, Urlaub H, Sheldrick GM, Gärtner J, Krätzner R
Nucleic Acids Res 40 8733-42 (2012)
PMID: 22735700

Function and Biology Details

Reaction catalysed: 
(1a) RNA = an (RNA fragment)-3'-nucleoside-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126360 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease T2 Chain: A
Molecule details ›
Chain: A
Length: 238 amino acids
Theoretical weight: 28.01 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O00584 (Residues: 25-256; Coverage: 100%)
Gene names: RNASE6PL, RNASET2
Sequence domains: Ribonuclease T2 family
Structure domains: Ribonuclease T2-like

Ligands and Environments

1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 31.2Å b: 67.4Å c: 47.9Å
α: 90° β: 90.81° γ: 90°
R-values:
R R work R free
0.154 0.152 0.19
Expression system: Homo sapiens

Quick links

Citations

8 review citations

Immune Modulation by Human Secreted RNases at the Extracellular Space.
Lu et al. (2018)
7 more

3 mentions without citation

Structure and activity of the only human RNase T2.
Thorn et al. (2012)
2 more