› 3sr3

X-ray diffraction
1.5Å resolution

Crystal structure of the w180a mutant of microcin immunity protein mccf from Bacillus anthracis shows the active site loop in the open conformation.

Released:
DOI: 10.2210/pdb3sr3/pdb
PDB entry 3sr3 coloured by chain, front view.
PDB entry 3sr3 coloured by chain, side view.
PDB entry 3sr3 coloured by chain, top view.
Source organism: Bacillus anthracis str. Ames
Primary publication:
Structural and functional characterization of microcin C resistance peptidase MccF from Bacillus anthracis.
Nocek B, Tikhonov A, Babnigg G, Gu M, Zhou M, Makarova KS, Vondenhoff G, Van Aerschot A, Kwon K, Anderson WF, Severinov K, Joachimiak A
J Mol Biol 420 366-83 (2012)
PMID: 22516613

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105739 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Microcin immunity protein MccF Chains: A, B
Molecule details ›
Chains: A, B
Length: 336 amino acids
Theoretical weight: 37.91 KDa
Source organism: Bacillus anthracis str. Ames
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A6L8PEJ7 (Residues: 1-333; Coverage: 100%)
Gene name: GBAA_1949
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 51.315Å b: 97.137Å c: 125.582Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.149 0.178
Expression system: Escherichia coli BL21(DE3)

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Citations

8 review citations

Bacteriocins - a viable alternative to antibiotics?
Cotter et al. (2013)
7 more

6 mentions without citation

Fast and anisotropic flexibility-rigidity index for protein flexibility and fluctuation analysis.
Opron et al. (2014)
5 more