› 3sev

X-ray diffraction
3.05Å resolution

Zn-mediated Trimer of Maltose-binding Protein E310H/K314H by Synthetic Symmetrization

Released:
DOI: 10.2210/pdb3sev/pdb
PDB entry 3sev coloured by chain, front view.
PDB entry 3sev coloured by chain, side view.
PDB entry 3sev coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
An approach to crystallizing proteins by metal-mediated synthetic symmetrization.
Laganowsky A, Zhao M, Soriaga AB, Sawaya MR, Cascio D, Yeates TO
Protein Sci 20 1876-90 (2011)
PMID: 21898649

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142641 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 372 amino acids
Theoretical weight: 40.64 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-384; Coverage: 97%)
Gene names: JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
2 bound ligands:
Ligand ZN 3 x ZN
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 113.78Å b: 115.72Å c: 119.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.221 0.263
Expression system: Escherichia coli

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Citations

9 review citations

The design of symmetric protein nanomaterials comes of age in theory and practice.
Yeates et al. (2016)
8 more