3ses

X-ray diffraction
1.9Å resolution

Cu-mediated Dimer of Maltose-binding Protein A216H/K220H by Synthetic Symmetrization

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-142613 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 372 amino acids
Theoretical weight: 40.7 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-384; Coverage: 97%)
Gene names: JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 72.85Å b: 61.25Å c: 89.45Å
α: 90° β: 109.71° γ: 90°
R-values:
R R work R free
0.173 0.172 0.205
Expression system: Escherichia coli