› 3s7n

X-ray diffraction
2.45Å resolution

Crystal Structure of the alternate His 207 conformation of the Infrared Fluorescent D207H variant of Deinococcus Bacteriophytochrome chromophore binding domain at 2.45 angstrom resolution

Released:
DOI: 10.2210/pdb3s7n/pdb
PDB entry 3s7n coloured by chain, front view.
PDB entry 3s7n coloured by chain, side view.
PDB entry 3s7n coloured by chain, top view.
Source organism: Deinococcus radiodurans
Primary publication:
Structure-guided engineering enhances a phytochrome-based infrared fluorescent protein.
Auldridge ME, Satyshur KA, Anstrom DM, Forest KT
J Biol Chem 287 7000-9 (2012)
PMID: 22210774

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193367 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriophytochrome Chain: A
Molecule details ›
Chain: A
Length: 343 amino acids
Theoretical weight: 37.18 KDa
Source organism: Deinococcus radiodurans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9RZA4 (Residues: 1-321; Coverage: 43%)
Gene names: DR_A0050, bphP
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand LBV 1 x LBV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: C2
Unit cell:
a: 96.413Å b: 52.808Å c: 74.801Å
α: 90° β: 113.13° γ: 90°
R-values:
R R work R free
0.178 0.174 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins.
Rodriguez et al. (2017)
13 more

1 mention without citation

Structure-guided engineering enhances a phytochrome-based infrared fluorescent protein.
Auldridge et al. (2012)