› 3s6y

X-ray diffraction
2.79Å resolution

Structure of reovirus attachment protein sigma1 in complex with alpha-2,6-sialyllactose

Released:
DOI: 10.2210/pdb3s6y/pdb
PDB entry 3s6y coloured by chain, front view.
PDB entry 3s6y coloured by chain, side view.
PDB entry 3s6y coloured by chain, top view.
Source organism: Mammalian orthoreovirus 3 Dearing
Primary publication:
Crystal structure of reovirus attachment protein σ1 in complex with sialylated oligosaccharides.
Reiter DM, Frierson JM, Halvorson EE, Kobayashi T, Dermody TS, Stehle T
PLoS Pathog 7 e1002166 (2011)
PMID: 21829363

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137113 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Outer capsid protein sigma-1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 325 amino acids
Theoretical weight: 35.36 KDa
Source organism: Mammalian orthoreovirus 3 Dearing
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03528 (Residues: 170-455; Coverage: 63%)
Gene name: S1
Sequence domains: Reovirus viral attachment protein sigma 1
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: SIA, BGC, GAL
Carbohydrate polymer
1 bound ligand:
Ligand SIA 1 x SIA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21212
Unit cell:
a: 87.15Å b: 333.18Å c: 58.49Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.158 0.208
Expression system: Escherichia coli BL21(DE3)

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Citations

18 review citations

Viral quasispecies evolution.
Domingo et al. (2012)
17 more

2 mentions without citation

Potential for Improving Potency and Specificity of Reovirus Oncolysis with Next-Generation Reovirus Variants.
Mohamed et al. (2015)
1 more