› 3s48

X-ray diffraction
3.05Å resolution

Human Alpha-Haemoglobin Complexed with the First NEAT Domain of IsdH from Staphylococcus aureus

Released:
DOI: 10.2210/pdb3s48/pdb
PDB entry 3s48 coloured by chain, front view.
PDB entry 3s48 coloured by chain, side view.
PDB entry 3s48 coloured by chain, top view.
Source organisms:
Primary publication:
The structure of α-haemoglobin in complex with a haemoglobin-binding domain from Staphylococcus aureus reveals the elusive α-haemoglobin dimerization interface.
Kumar KK, Jacques DA, Guss JM, Gell DA
Acta Crystallogr F Struct Biol Commun 70 1032-7 (2014)
PMID: 25084376

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159682 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Iron-regulated surface determinant protein H Chains: A, B
Molecule details ›
Chains: A, B
Length: 164 amino acids
Theoretical weight: 18.9 KDa
Source organism: Staphylococcus aureus subsp. aureus MSSA476
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6G8J7 (Residues: 86-229; Coverage: 17%)
Gene names: SAS1657, harA, isdH, sasI
Sequence domains: Iron Transport-associated domain
Structure domains: Immunoglobulin-like
Hemoglobin subunit alpha Chains: C, D
Molecule details ›
Chains: C, D
Length: 141 amino acids
Theoretical weight: 15.15 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P69905 (Residues: 2-142; Coverage: 99%)
Gene names: HBA1, HBA2
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

1 bound ligand:
Ligand HEM 2 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: I41
Unit cell:
a: 115.607Å b: 115.607Å c: 142.333Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.265 0.24 0.268
Expression system: Escherichia coli

Quick links

Citations

1 review citation

NEAr Transporter (NEAT) Domains: Unique Surface Displayed Heme Chaperones That Enable Gram-Positive Bacteria to Capture Heme-Iron From Hemoglobin.
Ellis-Guardiola et al. (2020)
4 other articles

1 mention without citation

The structure of α-haemoglobin in complex with a haemoglobin-binding domain from Staphylococcus aureus reveals the elusive α-haemoglobin dimerization interface.
Kumar et al. (2014)