› 3rst

X-ray diffraction
2.37Å resolution

Crystal structure of Bacillus subtilis signal peptide peptidase A

Released:
DOI: 10.2210/pdb3rst/pdb
PDB entry 3rst coloured by chain, front view.
PDB entry 3rst coloured by chain, side view.
PDB entry 3rst coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Crystal structure of Bacillus subtilis signal peptide peptidase A.
Nam SE, Kim AC, Paetzel M
J Mol Biol 419 347-58 (2012)
PMID: 22472423

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128551 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative signal peptide peptidase SppA Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 240 amino acids
Theoretical weight: 26.16 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O34525 (Residues: 56-295; Coverage: 72%)
Gene names: BSU29530, sppA, yteI
Sequence domains: Peptidase family S49
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 87.779Å b: 131.066Å c: 207.263Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.206 0.241
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

An overview of Bacillus proteases: from production to application.
Contesini et al. (2018)
3 more