3rpu

X-ray diffraction
3.6Å resolution

Crystal structure of the MukE-MukF complex

Released:
Source organism: Escherichia coli K-12
Primary publication:
The role of MukE in assembling a functional MukBEF complex.
J Mol Biol 412 578-90 (2011)
PMID: 21855551

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-149663 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chromosome partition protein MukF Chains: A, B, X
Molecule details ›
Chains: A, B, X
Length: 460 amino acids
Theoretical weight: 52.81 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P60293 (Residues: 1-440; Coverage: 100%)
Gene names: JW0905, b0922, kicB, mukF
Sequence domains:
Structure domains:
Chromosome partition protein MukE Chains: D, E, G, H, Y, Z
Molecule details ›
Chains: D, E, G, H, Y, Z
Length: 245 amino acids
Theoretical weight: 28.19 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22524 (Residues: 10-234; Coverage: 96%)
Gene names: JW0906, b0923, kicA, mukE, ycbA
Sequence domains: MukE-like family
Structure domains: Arc Repressor Mutant, subunit A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P6522
Unit cell:
a: 149.89Å b: 149.89Å c: 738.551Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.217 0.274
Expression system: Escherichia coli BL21(DE3)