› 3rko

X-ray diffraction
3Å resolution

Crystal structure of the membrane domain of respiratory complex I from E. coli at 3.0 angstrom resolution

Released:
DOI: 10.2210/pdb3rko/pdb
PDB entry 3rko coloured by chain, front view.
PDB entry 3rko coloured by chain, side view.
PDB entry 3rko coloured by chain, top view.
Source organism: Escherichia coli BL21(DE3)
Primary publication:
Structure of the membrane domain of respiratory complex I.
Efremov RG, Sazanov LA
Nature 476 414-20 (2011)
PMID: 21822288

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111535 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
NADH-QUINONE OXIDOREDUCTASE SUBUNIT L Chains: B, L
Molecule details ›
Chains: B, L
Length: 613 amino acids
Theoretical weight: 66.51 KDa
Source organism: Escherichia coli BL21(DE3)
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
NADH-QUINONE OXIDOREDUCTASE SUBUNIT M Chains: C, M
Molecule details ›
Chains: C, M
Length: 509 amino acids
Theoretical weight: 56.56 KDa
Source organism: Escherichia coli BL21(DE3)
NADH-QUINONE OXIDOREDUCTASE SUBUNIT N Chains: D, N
Molecule details ›
Chains: D, N
Length: 485 amino acids
Theoretical weight: 52.07 KDa
Source organism: Escherichia coli BL21(DE3)
NADH-quinone oxidoreductase subunit K Chains: G, K
Molecule details ›
Chains: G, K
Length: 100 amino acids
Theoretical weight: 10.85 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C5W716 (Residues: 1-100; Coverage: 100%)
Gene names: B21_02164, ECBD_1382, ECD_02204, nuoK
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase chain 4L
Structure domains: Helix Hairpins
NADH-QUINONE OXIDOREDUCTASE SUBUNIT A Chains: A, E
Molecule details ›
Chains: A, E
Length: 147 amino acids
Theoretical weight: 16.47 KDa
Source organism: Escherichia coli BL21(DE3)
Structure domains: NADH:ubiquinone/plastoquinone oxidoreductase, chain 3
NADH-QUINONE OXIDOREDUCTASE SUBUNIT J Chains: F, J
Molecule details ›
Chains: F, J
Length: 184 amino acids
Theoretical weight: 19.89 KDa
Source organism: Escherichia coli BL21(DE3)
Structure domains: NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ

Ligands and Environments

2 bound ligands:
Ligand LFA 18 x LFA
Ligand CA7 2 x CA7
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P1
Unit cell:
a: 94.98Å b: 116.57Å c: 191.37Å
α: 98.51° β: 104.08° γ: 108.51°
R-values:
R R work R free
0.233 0.232 0.282

Quick links

Citations

53 review citations

Mitochondrial complex I.
Hirst J. (2013)
52 more

45 mentions without citation

Genotype to phenotype: Diet-by-mitochondrial DNA haplotype interactions drive metabolic flexibility and organismal fitness.
Aw et al. (2018)
44 more