› 3qzo

X-ray diffraction
1.95Å resolution

Staphylococcus aureus IsdA NEAT domain in complex with heme, reduced crystal

Released:
DOI: 10.2210/pdb3qzo/pdb
PDB entry 3qzo coloured by chain, front view.
PDB entry 3qzo coloured by chain, side view.
PDB entry 3qzo coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus N315
Primary publication:
Iron-coordinating tyrosine is a key determinant of NEAT domain heme transfer.
Grigg JC, Mao CX, Murphy ME
J Mol Biol 413 684-98 (2011)
PMID: 21893067

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181330 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Iron-regulated surface determinant protein A Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 127 amino acids
Theoretical weight: 14.65 KDa
Source organism: Staphylococcus aureus subsp. aureus N315
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q7A655 (Residues: 62-184; Coverage: 41%)
Gene names: SA0977, frpA, isdA, stbA
Sequence domains: Iron Transport-associated domain
Structure domains: Immunoglobulin-like

Ligands and Environments

2 bound ligands:
Ligand HEM 4 x HEM
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P21
Unit cell:
a: 56.041Å b: 58.592Å c: 97.834Å
α: 90° β: 92.98° γ: 90°
R-values:
R R work R free
0.196 0.194 0.244
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Recent developments in understanding the iron acquisition strategies of gram positive pathogens.
Sheldon et al. (2015)
3 more

1 mention without citation

Extracellular heme uptake and the challenges of bacterial cell membranes.
Smith et al. (2012)