› 3qs4

X-ray diffraction
2.63Å resolution

Crystal structure of LeuT mutant F259V bound to sodium and L-tryptophan

Released:
DOI: 10.2210/pdb3qs4/pdb
PDB entry 3qs4 coloured by chain, front view.
PDB entry 3qs4 coloured by chain, side view.
PDB entry 3qs4 coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Insights into transport mechanism from LeuT engineered to transport tryptophan.
Piscitelli CL, Gouaux E
EMBO J 31 228-35 (2012)
PMID: 21952050

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-130575 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Na(+):neurotransmitter symporter (Snf family) Chain: A
Molecule details ›
Chain: A
Length: 519 amino acids
Theoretical weight: 58.03 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67854 (Residues: 1-513; Coverage: 100%)
Gene names: aq_2077, snf
Sequence domains: Sodium:neurotransmitter symporter family

Ligands and Environments

3 bound ligands:
Ligand TRP 3 x TRP
Ligand BOG 3 x BOG
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: C2
Unit cell:
a: 90.219Å b: 86.595Å c: 82.058Å
α: 90° β: 93.63° γ: 90°
R-values:
R R work R free
0.215 0.214 0.228
Expression system: Escherichia coli

Quick links

Citations

8 review citations

SLC6 transporters: structure, function, regulation, disease association and therapeutics.
Pramod et al. (2013)
7 more

2 mentions without citation

Insights into transport mechanism from LeuT engineered to transport tryptophan.
Piscitelli et al. (2012)
1 more