› 3qpu

X-ray diffraction
2.3Å resolution

PFKFB3 in complex with PPi

Released:
DOI: 10.2210/pdb3qpu/pdb
PDB entry 3qpu coloured by chain, front view.
PDB entry 3qpu coloured by chain, side view.
PDB entry 3qpu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis of the fructose-2,6-bisphosphatase reaction of PFKFB3: transition state and the C-terminal function.
Cavalier MC, Kim SG, Neau D, Lee YH
Proteins 80 1143-53 (2012)
PMID: 22275052

Function and Biology Details

Reactions catalysed: 
ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate
Beta-D-fructose 2,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172610 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 Chain: A
Molecule details ›
Chain: A
Length: 520 amino acids
Theoretical weight: 59.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16875 (Residues: 1-520; Coverage: 100%)
Gene name: PFKFB3
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand POP 3 x POP
Ligand SRT 2 x SRT
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P6522
Unit cell:
a: 102.975Å b: 102.975Å c: 258.128Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.184 0.236
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Role of PFKFB3 and PFKFB4 in Cancer: Genetic Basis, Impact on Disease Development/Progression, and Potential as Therapeutic Targets.
Kotowski et al. (2021)
2 more

1 mention without citation

Molecular basis of the fructose-2,6-bisphosphatase reaction of PFKFB3: transition state and the C-terminal function.
Cavalier et al. (2012)