› 3qpe

X-ray diffraction
1.8Å resolution

Crystal structure of Galacturonate Dehydratase from GEOBACILLUS SP. complexed with D-Galacturonate and 5-keto-4-deoxy-D-Galacturonate

Released:
DOI: 10.2210/pdb3qpe/pdb
PDB entry 3qpe coloured by chain, front view.
PDB entry 3qpe coloured by chain, side view.
PDB entry 3qpe coloured by chain, top view.
Source organism: Paenibacillus sp. Y412MC10
Entry authors: Fedorov AA, Fedorov EV, Mills-Groninger F, Gerlt JA, Almo SC

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112471 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mandelate racemase/muconate lactonizing enzyme C-terminal domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 392 amino acids
Theoretical weight: 43.92 KDa
Source organism: Paenibacillus sp. Y412MC10
Expression system: Escherichia coli
UniProt:
  • Canonical: D3EID5 (Residues: 2-382; Coverage: 100%)
Gene name: GYMC10_3367
Sequence domains: Enolase C-terminal domain-like
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MG 8 x MG
Ligand DGU 1 x DGU
Ligand D54 3 x D54
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 158.019Å b: 66.265Å c: 154.609Å
α: 90° β: 95.73° γ: 90°
R-values:
R R work R free
0.224 0.221 0.27
Expression system: Escherichia coli

Quick links