› 3qfd

X-ray diffraction
1.68Å resolution

Human Class I MHC HLA-A2 in complex with Mart-1(27-35) nonameric peptide

Released:
DOI: 10.2210/pdb3qfd/pdb
PDB entry 3qfd coloured by chain, front view.
PDB entry 3qfd coloured by chain, side view.
PDB entry 3qfd coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Loss of T cell antigen recognition arising from changes in peptide and major histocompatibility complex protein flexibility: implications for vaccine design.
Insaidoo FK, Borbulevych OY, Hossain M, Santhanagopolan SM, Baxter TK, Baker BM
J Biol Chem 286 40163-73 (2011)
PMID: 21937447

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-137805 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class I histocompatibility antigen, A alpha chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 275 amino acids
Theoretical weight: 31.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04439 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, E
Molecule details ›
Chains: B, E
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Melanoma antigen recognized by T-cells 1 Chains: C, F
Molecule details ›
Chains: C, F
Length: 9 amino acids
Theoretical weight: 814 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q16655 (Residues: 27-35; Coverage: 8%)
Gene names: MART1, MLANA

Ligands and Environments

2 bound ligands:
Ligand GOL 6 x GOL
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21
Unit cell:
a: 58.254Å b: 84.118Å c: 83.964Å
α: 90° β: 90.05° γ: 90°
R-values:
R R work R free
0.172 0.17 0.211
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

T cell antigen receptor recognition of antigen-presenting molecules.
Rossjohn et al. (2015)
6 more

5 mentions without citation

Loss of T cell antigen recognition arising from changes in peptide and major histocompatibility complex protein flexibility: implications for vaccine design.
Insaidoo et al. (2011)
4 more