› 3q3f

X-ray diffraction
2.17Å resolution

Engineering Domain-Swapped Binding Interfaces by Mutually Exclusive Folding: Insertion of Ubiquitin into position 103 of Barnase

Released:
DOI: 10.2210/pdb3q3f/pdb
PDB entry 3q3f coloured by chain, front view.
PDB entry 3q3f coloured by chain, side view.
PDB entry 3q3f coloured by chain, top view.
Source organisms:
Primary publication:
Engineering domain-swapped binding interfaces by mutually exclusive folding.
Ha JH, Karchin JM, Walker-Kopp N, Huang LS, Berry EA, Loh SN
J Mol Biol 416 495-502 (2012)
PMID: 22245575

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132800 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease; Ubiquitin Chain: A
Molecule details ›
Chain: A
Length: 189 amino acids
Theoretical weight: 21.13 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P00648 (Residues: 48-150, 151-151; Coverage: 85%)
  • Canonical: P0CG48 (Residues: 535-608, 610-614; Coverage: 12%)
Gene name: UBC
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 16 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P3221
Unit cell:
a: 86.872Å b: 86.872Å c: 75.571Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.184 0.233
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Protein conformational switches: from nature to design.
Ha et al. (2012)
1 more

2 mentions without citation

Engineering domain-swapped binding interfaces by mutually exclusive folding.
Ha et al. (2012)
1 more