› 3py6

X-ray diffraction
1.7Å resolution

Crystal Structure of a Beta-Lactamase-Like Protein from Brucella Melitensis bound to GMP

Released:
DOI: 10.2210/pdb3py6/pdb
PDB entry 3py6 coloured by chain, front view.
PDB entry 3py6 coloured by chain, side view.
PDB entry 3py6 coloured by chain, top view.
Source organism: Brucella abortus 2308
Primary publication:
BrabA.11339.a: anomalous diffraction and ligand binding guide towards the elucidation of the function of a 'putative β-lactamase-like protein' from Brucella melitensis.
Abendroth J, Sankaran B, Edwards TE, Gardberg AS, Dieterich S, Bhandari J, Napuli AJ, Van Voorhis WC, Staker BL, Myler PJ, Stewart LJ
Acta Crystallogr Sect F Struct Biol Cryst Commun 67 1106-12 (2011)
PMID: 21904058

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174184 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallo-beta-lactamase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 30.73 KDa
Source organism: Brucella abortus 2308
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2YQ74 (Residues: 1-272; Coverage: 100%)
Gene name: BAB1_1016
Sequence domains: Beta-lactamase superfamily domain
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

4 bound ligands:
Ligand MN 2 x MN
Ligand K 1 x K
Ligand 5GP 1 x 5GP
Ligand TAR 1 x TAR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: C2221
Unit cell:
a: 72.86Å b: 75.23Å c: 98.54Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.141 0.14 0.168
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

An unusual diphosphatase from the PhnP family cleaves reactive FAD photoproducts.
Beaudoin et al. (2018)

2 mentions without citation

Influence of C-H...O interactions on the structural stability of β-lactamases.
Lavanya et al. (2013)
1 more