› 3pgu

X-ray diffraction
1.7Å resolution

Phe3Glu mutant of EcFadL

Released:
DOI: 10.2210/pdb3pgu/pdb
PDB entry 3pgu coloured by chain, front view.
PDB entry 3pgu coloured by chain, side view.
PDB entry 3pgu coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Ligand-gated diffusion across the bacterial outer membrane.
Lepore BW, Indic M, Pham H, Hearn EM, Patel DR, van den Berg B
Proc Natl Acad Sci U S A 108 10121-6 (2011)
PMID: 21593406

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145291 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Long-chain fatty acid transport protein Chain: A
Molecule details ›
Chain: A
Length: 427 amino acids
Theoretical weight: 46.75 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P10384 (Residues: 26-446; Coverage: 100%)
Gene names: JW2341, b2344, fadL, ttr
Sequence domains: Outer membrane protein transport protein (OMPP1/FadL/TodX)
Structure domains: Outer membrane protein transport protein (OMPP1/FadL/TodX)

Ligands and Environments

5 bound ligands:
Ligand C8E 14 x C8E
Ligand 2PE 3 x 2PE
Ligand NI 2 x NI
Ligand NA 2 x NA
Ligand OLA 1 x OLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P41212
Unit cell:
a: 65.2Å b: 65.2Å c: 280.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.168 0.18
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Microbe-host interactions: structure and role of Gram-negative bacterial porins.
Galdiero et al. (2012)
4 more

1 mention without citation

Ligand-gated diffusion across the bacterial outer membrane.
Lepore et al. (2011)