› 3pf4

X-ray diffraction
1.38Å resolution

Crystal structure of Bs-CspB in complex with r(GUCUUUA)

Released:
DOI: 10.2210/pdb3pf4/pdb
PDB entry 3pf4 coloured by chain, front view.
PDB entry 3pf4 coloured by chain, side view.
PDB entry 3pf4 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
RNA single strands bind to a conserved surface of the major cold shock protein in crystals and solution.
Sachs R, Max KE, Heinemann U, Balbach J
RNA 18 65-76 (2012)
PMID: 22128343

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
hetero dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-152196 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Cold shock protein CspB Chains: A, B
Molecule details ›
Chains: A, B
Length: 67 amino acids
Theoretical weight: 7.37 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P32081 (Residues: 1-67; Coverage: 100%)
Gene names: BSU09100, cspA, cspB
Sequence domains: 'Cold-shock' DNA-binding domain
Structure domains: Nucleic acid-binding proteins
hexaribonucleotide (rGUCUUUA) Chain: R
Molecule details ›
Chain: R
Length: 7 nucleotides
Theoretical weight: 2.16 KDa
Source organism: Bacillus subtilis
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand NA 1 x NA
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P212121
Unit cell:
a: 49.275Å b: 50.512Å c: 57.802Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.154 0.194
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

7 review citations

How RNA-Binding Proteins Interact with RNA: Molecules and Mechanisms.
Corley et al. (2020)
6 more

10 mentions without citation

Mechanisms of Lin28-mediated miRNA and mRNA regulation--a structural and functional perspective.
Mayr et al. (2013)
9 more