› 3pe7

X-ray diffraction
1.65Å resolution

Oligogalacturonate lyase in complex with manganese

Released:
DOI: 10.2210/pdb3pe7/pdb
PDB entry 3pe7 coloured by chain, front view.
PDB entry 3pe7 coloured by chain, side view.
PDB entry 3pe7 coloured by chain, top view.
Source organism: Yersinia enterocolitica subsp. enterocolitica 8081
Primary publication:
The active site of oligogalacturonate lyase provides unique insights into cytoplasmic oligogalacturonate beta-elimination.
Abbott DW, Gilbert HJ, Boraston AB
J Biol Chem 285 39029-38 (2010)
PMID: 20851883

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106623 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oligogalacturonate lyase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 388 amino acids
Theoretical weight: 44.26 KDa
Source organism: Yersinia enterocolitica subsp. enterocolitica 8081
Expression system: Escherichia coli
UniProt:
  • Canonical: A1JMA5 (Residues: 1-388; Coverage: 100%)
Gene names: YE1876, ogl
Sequence domains: Oligogalacturonate lyase
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase

Ligands and Environments

3 bound ligands:
Ligand MN 1 x MN
Ligand CA 2 x CA
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 66.65Å b: 75.51Å c: 78.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.177 0.207
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Bacterial pectate lyases, structural and functional diversity.
Hugouvieux-Cotte-Pattat et al. (2014)
2 more

2 mentions without citation

The active site of oligogalacturonate lyase provides unique insights into cytoplasmic oligogalacturonate beta-elimination.
Abbott et al. (2010)
1 more