› 3p9p

X-ray diffraction
1.5Å resolution

Structure of I274V variant of E. coli KatE

Released:
DOI: 10.2210/pdb3p9p/pdb
PDB entry 3p9p coloured by chain, front view.
PDB entry 3p9p coloured by chain, side view.
PDB entry 3p9p coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Modulation of heme orientation and binding by a single residue in catalase HPII of Escherichia coli.
Jha V, Louis S, Chelikani P, Carpena X, Donald LJ, Fita I, Loewen PC
Biochemistry 50 2101-10 (2011)
PMID: 21332158

Function and Biology Details

Reaction catalysed: 
2 H(2)O(2) = O(2) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149122 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Catalase HPII Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 753 amino acids
Theoretical weight: 84.26 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P21179 (Residues: 1-753; Coverage: 100%)
Gene names: JW1721, b1732, katE
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 8 x HEM

Cofactor: Ligand HDE 4 x HDE

Cofactor: Ligand HDD 4 x HDD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P21
Unit cell:
a: 93.5Å b: 132.82Å c: 122.59Å
α: 90° β: 109.47° γ: 90°
R-values:
R R work R free
0.15 0.149 0.185
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Thirty years of heme catalases structural biology.
Díaz et al. (2012)
2 more

1 mention without citation

JLigand: a graphical tool for the CCP4 template-restraint library.
Lebedev et al. (2012)