› 3p7z

X-ray diffraction
2.65Å resolution

Crystal structure of the Neurofibromin Sec14-PH module containing the patient derived mutation I1584V

Released:
DOI: 10.2210/pdb3p7z/pdb
PDB entry 3p7z coloured by chain, front view.
PDB entry 3p7z coloured by chain, side view.
PDB entry 3p7z coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and biochemical consequences of NF1 associated nontruncating mutations in the Sec14-PH module of neurofibromin.
Welti S, Kühn S, D'Angelo I, Brügger B, Kaufmann D, Scheffzek K
Hum Mutat 32 191-7 (2011)
PMID: 21089070

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149214 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neurofibromin Chains: A, B
Molecule details ›
Chains: A, B
Length: 276 amino acids
Theoretical weight: 31.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P21359 (Residues: 1566-1837; Coverage: 10%)
Gene name: NF1
Sequence domains: Divergent CRAL/TRIO domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand PEV 2 x PEV
Ligand NA 1 x NA
Ligand POP 2 x POP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P41212
Unit cell:
a: 113.061Å b: 113.061Å c: 124.612Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.216 0.266
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Molecular mechanisms promoting the pathogenesis of Schwann cell neoplasms.
Carroll SL. (2012)
3 more

4 mentions without citation

An N-terminal Amphipathic Helix Binds Phosphoinositides and Enhances Kalirin Sec14 Domain-mediated Membrane Interactions.
Miller et al. (2015)
3 more