› 3p6m

X-ray diffraction
2Å resolution

Crystal Structure of Cytochrome P450cam crystallized in the presence of a tethered substrate analog AdaC1-C8-Dans

Released:
DOI: 10.2210/pdb3p6m/pdb
PDB entry 3p6m coloured by chain, front view.
PDB entry 3p6m coloured by chain, side view.
PDB entry 3p6m coloured by chain, top view.
Source organism: Pseudomonas putida
Entry authors: Lee Y-T, Wilson RF, Glazer EC, Goodin DB

Function and Biology Details

Reaction catalysed: 
(+)-camphor + reduced putidaredoxin + O(2) = (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132314 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Camphor 5-monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 414 amino acids
Theoretical weight: 46.56 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: P00183 (Residues: 2-415; Coverage: 100%)
Gene names: camC, cyp101
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
2 bound ligands:
Ligand DSO 1 x DSO
Ligand K 1 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P212121
Unit cell:
a: 65.29Å b: 74.6Å c: 92.36Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.251
Expression system: Escherichia coli

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