› 3ouz

X-ray diffraction
1.9Å resolution

Crystal Structure of Biotin Carboxylase-ADP complex from Campylobacter jejuni

Released:
DOI: 10.2210/pdb3ouz/pdb
PDB entry 3ouz coloured by chain, front view.
PDB entry 3ouz coloured by chain, side view.
PDB entry 3ouz coloured by chain, top view.
Source organism: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
Entry authors: Maltseva N, Kim Y, Makowska-Grzyska M, Mulligan R, Papazisi L, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
ATP + [biotin carboxyl-carrier protein]-biotin-N(6)-L-lysine + hydrogencarbonate- = ADP + phosphate + [biotin carboxyl-carrier protein]-carboxybiotin-N(6)-L-lysine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-171023 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Biotin carboxylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 446 amino acids
Theoretical weight: 50.27 KDa
Source organism: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0P8W7 (Residues: 1-443; Coverage: 100%)
Gene names: Cj1290c, accC
Sequence domains:
Structure domains:

Ligands and Environments

7 bound ligands:
Ligand GOL 13 x GOL
Ligand ADP 2 x ADP
Ligand MG 2 x MG
Ligand FMT 5 x FMT
Ligand SRT 1 x SRT
Ligand MLT 1 x MLT
Ligand TLA 1 x TLA
1 modified residue:
Ligand MSE 36 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 89.855Å b: 100.674Å c: 147.702Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.15 0.181
Expression system: Escherichia coli

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