› 3o4x

X-ray diffraction
3.2Å resolution

Crystal structure of complex between amino and carboxy terminal fragments of mDia1

Released:
DOI: 10.2210/pdb3o4x/pdb
PDB entry 3o4x coloured by chain, front view.
PDB entry 3o4x coloured by chain, side view.
PDB entry 3o4x coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of a complex between amino and carboxy terminal fragments of mDia1: insights into autoinhibition of diaphanous-related formins.
Nezami A, Poy F, Toms A, Zheng W, Eck MJ
PLoS One 5 (2010)
PMID: 20927338

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126686 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein diaphanous homolog 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 330 amino acids
Theoretical weight: 37.99 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O08808 (Residues: 131-458; Coverage: 26%)
Gene names: Diap1, Diaph1
Sequence domains:
Structure domains:
Protein diaphanous homolog 1 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 467 amino acids
Theoretical weight: 53.87 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O08808 (Residues: 736-1200; Coverage: 37%)
Gene names: Diap1, Diaph1
Sequence domains: Formin Homology 2 Domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 95.762Å b: 206.832Å c: 131.065Å
α: 90° β: 105.94° γ: 90°
R-values:
R R work R free
0.236 0.233 0.298
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Formins at a glance.
Breitsprecher et al. (2013)
6 more

4 mentions without citation

Formin and capping protein together embrace the actin filament in a ménage à trois.
Shekhar et al. (2015)
3 more