› 3o32

X-ray diffraction
2.85Å resolution

Crystal Structure of 4-Chlorocatechol Dioxygenase from Rhodococcus opacus 1CP in complex with 3,5-dichlorocatechol

Released:
DOI: 10.2210/pdb3o32/pdb
PDB entry 3o32 coloured by chain, front view.
PDB entry 3o32 coloured by chain, side view.
PDB entry 3o32 coloured by chain, top view.
Source organism: Rhodococcus opacus
Primary publication:
X-ray structures of 4-chlorocatechol 1,2-dioxygenase adducts with substituted catechols: new perspectives in the molecular basis of intradiol ring cleaving dioxygenases specificity.
Ferraroni M, Kolomytseva M, Scozzafava A, Golovleva L, Briganti F
J Struct Biol 181 274-82 (2013)
PMID: 23261399

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130594 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chlorocatechol 1,2-dioxygenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 257 amino acids
Theoretical weight: 28.98 KDa
Source organism: Rhodococcus opacus
UniProt:
  • Canonical: O67987 (Residues: 1-257; Coverage: 100%)
Gene name: clcA
Sequence domains:
Structure domains: Aromatic compound dioxygenase

Ligands and Environments

3 bound ligands:
Ligand FE 2 x FE
Ligand 35C 1 x 35C
Ligand MYY 2 x MYY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P6322
Unit cell:
a: 90.53Å b: 90.53Å c: 309.83Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.216 0.213 0.288

Quick links

Citations

4 citation in other articles

Benzoate degradation by Rhodococcus opacus 1CP after dormancy: Characterization of dioxygenases involved in the process.
Solyanikova et al. (2016)
3 more

1 mention without citation

Gamma-alumina: a single-crystal X-ray diffraction study.
Smrcok et al. (2006)