› 3o0z

X-ray diffraction
2.33Å resolution

Crystal structure of a coiled-coil domain from human ROCK I

Released:
DOI: 10.2210/pdb3o0z/pdb
PDB entry 3o0z coloured by chain, front view.
PDB entry 3o0z coloured by chain, side view.
PDB entry 3o0z coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of a coiled-coil domain from human ROCK I.
Tu D, Li Y, Song HK, Toms AV, Gould CJ, Ficarro SB, Marto JA, Goode BL, Eck MJ
PLoS One 6 e18080 (2011)
PMID: 21445309

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171700 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rho-associated protein kinase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 168 amino acids
Theoretical weight: 19.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13464 (Residues: 535-700; Coverage: 12%)
Gene name: ROCK1
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P43212
Unit cell:
a: 84.588Å b: 84.588Å c: 340.129Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.239 0.286
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Rho-associated kinases in tumorigenesis: re-considering ROCK inhibition for cancer therapy.
Rath et al. (2012)
3 more

8 mentions without citation

Crystal structure of a coiled-coil domain from human ROCK I.
Tu et al. (2011)
7 more