› 3o01

X-ray diffraction
1.9Å resolution

The Crystal Structure of the Salmonella Type III Secretion System Tip Protein SipD in Complex with Deoxycholate

Released:
DOI: 10.2210/pdb3o01/pdb
PDB entry 3o01 coloured by chain, front view.
PDB entry 3o01 coloured by chain, side view.
PDB entry 3o01 coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Primary publication:
The crystal structures of the Salmonella type III secretion system tip protein SipD in complex with deoxycholate and chenodeoxycholate.
Chatterjee S, Zhong D, Nordhues BA, Battaile KP, Lovell S, De Guzman RN
Protein Sci 20 75-86 (2011)
PMID: 21031487

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176315 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cell invasion protein SipD Chains: A, B
Molecule details ›
Chains: A, B
Length: 308 amino acids
Theoretical weight: 33.45 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli
UniProt:
  • Canonical: Q56026 (Residues: 39-343; Coverage: 89%)
Gene names: STM2883, sipD, sspD
Sequence domains: Type III secretion systems tip complex components
Structure domains: IpaD-like

Ligands and Environments

2 bound ligands:
Ligand NI 1 x NI
Ligand DXC 1 x DXC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-BM
Spacegroup: C2
Unit cell:
a: 201.931Å b: 52.327Å c: 57.311Å
α: 90° β: 90.34° γ: 90°
R-values:
R R work R free
0.199 0.196 0.257
Expression system: Escherichia coli

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Citations

15 review citations

Protein export according to schedule: architecture, assembly, and regulation of type III secretion systems from plant- and animal-pathogenic bacteria.
Büttner D. (2012)
14 more

5 mentions without citation

The crystal structures of the Salmonella type III secretion system tip protein SipD in complex with deoxycholate and chenodeoxycholate.
Chatterjee et al. (2011)
4 more