› 3nsb

X-ray diffraction
1.78Å resolution

Structure of bacteriorhodopsin ground state before and after X-ray modification

Released:
DOI: 10.2210/pdb3nsb/pdb
PDB entry 3nsb coloured by chain, front view.
PDB entry 3nsb coloured by chain, side view.
PDB entry 3nsb coloured by chain, top view.
Source organism: Halobacterium salinarum
Primary publication:
X-ray-radiation-induced changes in bacteriorhodopsin structure.
Borshchevskiy VI, Round ES, Popov AN, Büldt G, Gordeliy VI
J Mol Biol 409 813-25 (2011)
PMID: 21530535

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136413 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriorhodopsin Chain: A
Molecule details ›
Chain: A
Length: 248 amino acids
Theoretical weight: 26.81 KDa
Source organism: Halobacterium salinarum
UniProt:
  • Canonical: P02945 (Residues: 14-261; Coverage: 95%)
Gene names: VNG_1467G, bop
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

2 bound ligands:
Ligand RET 1 x RET
Ligand LI1 9 x LI1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P63
Unit cell:
a: 60.995Å b: 60.995Å c: 109.991Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.209 0.225

Quick links

Citations

4 review citations

Bacteriorhodopsin: Would the real structural intermediates please stand up?
Wickstrand et al. (2015)
3 more

3 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
2 more