› 3nnh

X-ray diffraction
2.75Å resolution

Crystal Structure of the CUGBP1 RRM1 with GUUGUUUUGUUU RNA

Released:
DOI: 10.2210/pdb3nnh/pdb
PDB entry 3nnh coloured by chain, front view.
PDB entry 3nnh coloured by chain, side view.
PDB entry 3nnh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into RNA recognition by the alternate-splicing regulator CUG-binding protein 1.
Teplova M, Song J, Gaw HY, Teplov A, Patel DJ
Structure 18 1364-77 (2010)
PMID: 20947024

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187796 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
CUGBP Elav-like family member 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 88 amino acids
Theoretical weight: 10.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92879 (Residues: 14-100; Coverage: 18%)
Gene names: BRUNOL2, CELF1, CUGBP, CUGBP1, NAB50
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
RNA (5'-R(*GP*UP*UP*GP*UP*UP*UP*UP*GP*UP*UP*U)-3') Chains: E, F
Molecule details ›
Chains: E, F
Length: 12 nucleotides
Theoretical weight: 3.75 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 59.18Å b: 62.074Å c: 122.052Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.183 0.263
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

12 review citations

The importance of CELF control: molecular and biological roles of the CUG-BP, Elav-like family of RNA-binding proteins.
Dasgupta et al. (2012)
11 more

6 mentions without citation

mRNA localization in metazoans: A structural perspective.
Lazzaretti et al. (2017)
5 more