› 3nm6

X-ray diffraction
1.6Å resolution

Helicobacter pylori MTAN complexed with adenine and tris

Released:
DOI: 10.2210/pdb3nm6/pdb
PDB entry 3nm6 coloured by chain, front view.
PDB entry 3nm6 coloured by chain, side view.
PDB entry 3nm6 coloured by chain, top view.
Source organism: Helicobacter pylori J99
Primary publication:
Enzyme-ligand interactions that drive active site rearrangements in the Helicobacter pylori 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase.
Ronning DR, Iacopelli NM, Mishra V
Protein Sci 19 2498-510 (2010)
PMID: 20954236

Function and Biology Details

Reactions catalysed: 
6-amino-6-deoxyfutalosine + H(2)O = dehypoxanthine futalosine + adenine
5'-deoxyadenosine + H(2)O = 5-deoxy-D-ribose + adenine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195684 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminodeoxyfutalosine nucleosidase Chain: B
Molecule details ›
Chain: B
Length: 230 amino acids
Theoretical weight: 25.05 KDa
Source organism: Helicobacter pylori J99
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ZMY2 (Residues: 1-230; Coverage: 100%)
Gene names: jhp_0082, mtn, mtnN
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

3 bound ligands:
Ligand ADE 1 x ADE
Ligand TRS 1 x TRS
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P3221
Unit cell:
a: 81.35Å b: 81.35Å c: 67.595Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.143 0.141 0.171
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Small molecule control of bacterial biofilms.
Worthington et al. (2012)
3 more

2 mentions without citation

Enzyme-ligand interactions that drive active site rearrangements in the Helicobacter pylori 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase.
Ronning et al. (2010)
1 more