› 3nl0

X-ray diffraction
2.6Å resolution

Mutant P44S M296I of Foot-and-mouth disease Virus RNA-dependent RNA polymerase

Released:
DOI: 10.2210/pdb3nl0/pdb
PDB entry 3nl0 coloured by chain, front view.
PDB entry 3nl0 coloured by chain, side view.
PDB entry 3nl0 coloured by chain, top view.
Source organism: Foot and mouth disease virus C
Primary publication:
A multi-step process of viral adaptation to a mutagenic nucleoside analogue by modulation of transition types leads to extinction-escape.
Agudo R, Ferrer-Orta C, Arias A, de la Higuera I, Perales C, Pérez-Luque R, Verdaguer N, Domingo E
PLoS Pathog 6 e1001072 (2010)
PMID: 20865120

Function and Biology Details

Reactions catalysed: 
Autocatalytically cleaves itself from the polyprotein of the foot-and-mouth disease virus by hydrolysis of a Lys-|-Gly bond, but then cleaves host cell initiation factor eIF-4G at bonds -Gly-|-Arg- and -Lys-|-Arg-.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192998 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
3D polymerase Chain: A
Molecule details ›
Chain: A
Length: 475 amino acids
Theoretical weight: 53.26 KDa
Source organism: Foot and mouth disease virus C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9QCE4 (Residues: 1858-2327; Coverage: 20%)
Sequence domains: Viral RNA-dependent RNA polymerase
Structure domains:
5'-R(P*UP*GP*GP*GP*CP*CP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 7 nucleotides
Theoretical weight: 2.21 KDa
Source organism: Foot and mouth disease virus C
Expression system: Not provided
5'-R(*G*GP*GP*CP*CP*C)-3' Chain: C
Molecule details ›
Chain: C
Length: 6 nucleotides
Theoretical weight: 1.91 KDa
Source organism: Foot and mouth disease virus C
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P3221
Unit cell:
a: 93.713Å b: 93.713Å c: 99.723Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.226 0.274
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

19 review citations

Viral quasispecies evolution.
Domingo et al. (2012)
18 more

1 mention without citation

Structure and Dynamics of a 197 bp Nucleosome in Complex with Linker Histone H1.
Bednar et al. (2017)