› 3ng6

X-ray diffraction
2.2Å resolution

The crystal structure of hemoglobin I from Trematomus newnesi in deoxygenated state obtained through an oxidation/reduction cycle in which potassium hexacyanoferrate and sodium dithionite were alternatively added

Released:
DOI: 10.2210/pdb3ng6/pdb
PDB entry 3ng6 coloured by chain, front view.
PDB entry 3ng6 coloured by chain, side view.
PDB entry 3ng6 coloured by chain, top view.
Source organism: Trematomus newnesi
Primary publication:
An order-disorder transition plays a role in switching off the root effect in fish hemoglobins.
Vergara A, Vitagliano L, Merlino A, Sica F, Marino K, Verde C, di Prisco G, Mazzarella L
J Biol Chem 285 32568-75 (2010)
PMID: 20610398

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155333 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Hemoglobin subunit alpha-1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 143 amino acids
Theoretical weight: 15.7 KDa
Source organism: Trematomus newnesi
UniProt:
  • Canonical: P45718 (Residues: 1-142; Coverage: 100%)
Gene name: hba1
Sequence domains: Globin
Structure domains: Globins
Hemoglobin subunit beta-1/2 Chains: B, D
Molecule details ›
Chains: B, D
Length: 146 amino acids
Theoretical weight: 16.25 KDa
Source organism: Trematomus newnesi
UniProt:
  • Canonical: P45720 (Residues: 1-146; Coverage: 100%)
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

1 bound ligand:
Ligand HEM 4 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P41
Unit cell:
a: 61.882Å b: 61.882Å c: 187.014Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.247

Quick links

Citations

1 review citation

An overview of biological macromolecule crystallization.
Russo Krauss et al. (2013)
14 other articles