› 3mzt

X-ray diffraction
2.7Å resolution

Protein-induced photophysical changes to the amyloid indicator dye, thioflavin T

Released:
DOI: 10.2210/pdb3mzt/pdb
PDB entry 3mzt coloured by chain, front view.
PDB entry 3mzt coloured by chain, side view.
PDB entry 3mzt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Protein-induced photophysical changes to the amyloid indicator dye thioflavin T.
Wolfe LS, Calabrese MF, Nath A, Blaho DV, Miranker AD, Xiong Y
Proc Natl Acad Sci U S A 107 16863-8 (2010)
PMID: 20826442

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158314 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-2-microglobulin Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 100 amino acids
Theoretical weight: 11.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand CU 6 x CU
Ligand TFX 3 x TFX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21212
Unit cell:
a: 92.891Å b: 116.592Å c: 66.317Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.239 0.256
Expression system: Escherichia coli

Quick links

Citations

14 review citations

ThT 101: a primer on the use of thioflavin T to investigate amyloid formation.
Gade Malmos et al. (2017)
13 more

1 mention without citation

Protein-induced photophysical changes to the amyloid indicator dye thioflavin T.
Wolfe et al. (2010)