› 3mwk

X-ray diffraction
1.45Å resolution

Q28E mutant of HERA N-terminal RecA-like domain, complex with 8-oxo-AMP

Released:
DOI: 10.2210/pdb3mwk/pdb
PDB entry 3mwk coloured by chain, front view.
PDB entry 3mwk coloured by chain, side view.
PDB entry 3mwk coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
Changing nucleotide specificity of the DEAD-box helicase Hera abrogates communication between the Q-motif and the P-loop.
Strohmeier J, Hertel I, Diederichsen U, Rudolph MG, Klostermeier D
Biol Chem 392 357-69 (2011)
PMID: 21391900

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180806 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heat resistant RNA dependent ATPase Chains: A, B
Molecule details ›
Chains: A, B
Length: 207 amino acids
Theoretical weight: 22.34 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q72GF3 (Residues: 8-214; Coverage: 40%)
Gene name: TT_C1895
Sequence domains: DEAD/DEAH box helicase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand 8OP 1 x 8OP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P41212
Unit cell:
a: 91.916Å b: 91.916Å c: 108.006Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.158 0.209
Expression system: Escherichia coli

Quick links

Citations

4 review citations

From unwinding to clamping - the DEAD box RNA helicase family.
Linder et al. (2011)
3 more