› 3mte

X-ray diffraction
1.8Å resolution

Crystal Structure of 16S rRNA Methyltranferase

Released:
DOI: 10.2210/pdb3mte/pdb
PDB entry 3mte coloured by chain, front view.
PDB entry 3mte coloured by chain, side view.
PDB entry 3mte coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural insights into the function of aminoglycoside-resistance A1408 16S rRNA methyltransferases from antibiotic-producing and human pathogenic bacteria.
Macmaster R, Zelinskaya N, Savic M, Rankin CR, Conn GL
Nucleic Acids Res 38 7791-9 (2010)
PMID: 20639535

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + adenine(1408) in 16S rRNA = S-adenosyl-L-homocysteine + N(1)-methyladenine(1408) in 16S rRNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108594 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
16S rRNA (adenine(1408)-N(1))-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 222 amino acids
Theoretical weight: 25.2 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A8C927 (Residues: 1-219; Coverage: 100%)
Gene name: npmA
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SAM 2 x SAM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P21
Unit cell:
a: 49.651Å b: 59.816Å c: 91.697Å
α: 90° β: 96.29° γ: 90°
R-values:
R R work R free
0.174 0.173 0.204
Expression system: Escherichia coli BL21

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Citations

2 review citations

16S rRNA Methyltransferases as Novel Drug Targets Against Tuberculosis.
Salaikumaran et al. (2022)
1 more

7 mentions without citation

Tied up in knots: Untangling substrate recognition by the SPOUT methyltransferases.
Strassler et al. (2022)
6 more