› 3mmv

X-ray diffraction
2.8Å resolution

Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation

Released:
DOI: 10.2210/pdb3mmv/pdb
PDB entry 3mmv coloured by chain, front view.
PDB entry 3mmv coloured by chain, side view.
PDB entry 3mmv coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structures of actin-bound Wiskott-Aldrich syndrome protein homology 2 (WH2) domains of Spire and the implication for filament nucleation.
Ducka AM, Joel P, Popowicz GM, Trybus KM, Schleicher M, Noegel AA, Huber R, Holak TA, Sitar T
Proc Natl Acad Sci U S A 107 11757-62 (2010)
PMID: 20538977

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145606 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin-5C Chain: A
Molecule details ›
Chain: A
Length: 374 amino acids
Theoretical weight: 41.72 KDa
Source organism: Drosophila melanogaster
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P10987 (Residues: 3-376; Coverage: 100%)
Gene names: Act5C, CG4027
Sequence domains: Actin
Structure domains:
Protein spire Chain: X
Molecule details ›
Chain: X
Length: 19 amino acids
Theoretical weight: 1.64 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P65
Unit cell:
a: 125.51Å b: 125.51Å c: 56.1Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.245 0.241 0.33
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

4 review citations

New mechanisms and functions of actin nucleation.
Firat-Karalar et al. (2011)
3 more

3 mentions without citation

A bipolar spindle of antiparallel ParM filaments drives bacterial plasmid segregation.
Gayathri et al. (2012)
2 more