› 3m9k

X-ray diffraction
1.5Å resolution

Crystal structure of human thioredoxin C69/73S double-mutant, oxidized form

Released:
DOI: 10.2210/pdb3m9k/pdb
PDB entry 3m9k coloured by chain, front view.
PDB entry 3m9k coloured by chain, side view.
PDB entry 3m9k coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human thioredoxin revealing an unraveled helix and exposed S-nitrosation site.
Weichsel A, Kem M, Montfort WR
Protein Sci 19 1801-6 (2010)
PMID: 20662007

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-145348 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin Chains: A, B
Molecule details ›
Chains: A, B
Length: 105 amino acids
Theoretical weight: 11.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P10599 (Residues: 1-105; Coverage: 100%)
Gene names: TRDX, TRX, TRX1, TXN
Sequence domains: Thioredoxin
Structure domains: Glutaredoxin

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand D1D 1 x D1D
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P6
Unit cell:
a: 125.62Å b: 125.62Å c: 33.55Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.184 0.211
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

The thioredoxin/peroxiredoxin/sulfiredoxin system: current overview on its redox function in plants and regulation by reactive oxygen and nitrogen species.
Sevilla et al. (2015)
2 more

3 mentions without citation

Thiol-Disulfide Exchange Reactions in the Mammalian Extracellular Environment.
Yi et al. (2016)
2 more