› 3lx2

X-ray diffraction
2.4Å resolution

Crystal Structure analysis of PCNA from Thermococcus kodakaraensis tk0582

Released:
DOI: 10.2210/pdb3lx2/pdb
PDB entry 3lx2 coloured by chain, front view.
PDB entry 3lx2 coloured by chain, side view.
PDB entry 3lx2 coloured by chain, top view.
Source organism: Thermococcus kodakarensis
Primary publication:
Crystal structures of two active proliferating cell nuclear antigens (PCNAs) encoded by Thermococcus kodakaraensis.
Ladner JE, Pan M, Hurwitz J, Kelman Z
Proc Natl Acad Sci U S A 108 2711-6 (2011)
PMID: 21270332

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177287 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase sliding clamp 2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 259 amino acids
Theoretical weight: 29.31 KDa
Source organism: Thermococcus kodakarensis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5JFD3 (Residues: 1-253; Coverage: 100%)
Gene names: TK0582, pcn2
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen

Ligands and Environments

1 bound ligand:
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: C2221
Unit cell:
a: 120.48Å b: 136.27Å c: 123.97Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.243 0.305
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Rapid progress of DNA replication studies in Archaea, the third domain of life.
Ishino et al. (2012)
7 more

4 mentions without citation

Crystal structures of two active proliferating cell nuclear antigens (PCNAs) encoded by Thermococcus kodakaraensis.
Ladner et al. (2011)
3 more