› 3lvv

X-ray diffraction
2.2Å resolution

BSO-inhibited ScGCL

Released:
DOI: 10.2210/pdb3lvv/pdb
PDB entry 3lvv coloured by chain, front view.
PDB entry 3lvv coloured by chain, side view.
PDB entry 3lvv coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural basis for feedback and pharmacological inhibition of Saccharomyces cerevisiae glutamate cysteine ligase.
Biterova EI, Barycki JJ
J Biol Chem 285 14459-66 (2010)
PMID: 20220146

Function and Biology Details

Reaction catalysed: 
ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152324 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate--cysteine ligase Chain: A
Molecule details ›
Chain: A
Length: 692 amino acids
Theoretical weight: 79.93 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P32477 (Residues: 1-678; Coverage: 100%)
Gene names: GSH1, J0832, YJL101C
Sequence domains: Glutamate-cysteine ligase
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand LBP 1 x LBP
Ligand ADP 1 x ADP
Ligand MG 3 x MG
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P43212
Unit cell:
a: 117.863Å b: 117.863Å c: 165.562Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.239
Expression system: Escherichia coli

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Citations

2 review citations

Transporter engineering in biomass utilization by yeast.
Hara et al. (2017)
1 more

3 mentions without citation

Structural basis for feedback and pharmacological inhibition of Saccharomyces cerevisiae glutamate cysteine ligase.
Biterova et al. (2010)
2 more