› 3lpq

X-ray diffraction
1.7Å resolution

Human MitoNEET with 2Fe-2S Coordinating Ligand His 87 Replaced With Cys

Released:
DOI: 10.2210/pdb3lpq/pdb
PDB entry 3lpq coloured by chain, front view.
PDB entry 3lpq coloured by chain, side view.
PDB entry 3lpq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mutation of the His ligand in mitoNEET stabilizes the 2Fe-2S cluster despite conformational heterogeneity in the ligand environment.
Conlan AR, Paddock ML, Homer C, Axelrod HL, Cohen AE, Abresch EC, Zuris JA, Nechushtai R, Jennings PA
Acta Crystallogr D Biol Crystallogr 67 516-23 (2011)
PMID: 21636891

Function and Biology Details

Reaction catalysed: 
L-cysteine + 2-oxoglutarate = 2-oxo-3-sulfanylpropanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192731 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CDGSH iron-sulfur domain-containing protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 79 amino acids
Theoretical weight: 9.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NZ45 (Residues: 33-107; Coverage: 69%)
Gene names: C10orf70, CISD1, MDS029, ZCD1
Sequence domains: Iron-binding zinc finger CDGSH type

Ligands and Environments

1 bound ligand:
Ligand FES 2 x FES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1, SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 49.311Å b: 49.587Å c: 59.222Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.178 0.226
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

mitoNEET as a novel drug target for mitochondrial dysfunction.
Geldenhuys et al. (2014)
2 more

5 mentions without citation

Engineering the redox potential over a wide range within a new class of FeS proteins.
Zuris et al. (2010)
4 more