› 3ll9

X-ray diffraction
2.15Å resolution

X-ray structures of isopentenyl phosphate kinase

Released:
DOI: 10.2210/pdb3ll9/pdb
PDB entry 3ll9 coloured by chain, front view.
PDB entry 3ll9 coloured by chain, side view.
PDB entry 3ll9 coloured by chain, top view.
Source organism: Methanothermobacter thermautotrophicus
Primary publication:
X-ray structures of isopentenyl phosphate kinase.
Mabanglo MF, Schubert HL, Chen M, Hill CP, Poulter CD
ACS Chem Biol 5 517-27 (2010)
PMID: 20402538

Function and Biology Details

Reaction catalysed: 
ATP + 3-methylbut-3-en-1-yl phosphate = ADP + 3-methylbut-3-en-1-yl diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127820 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isopentenyl phosphate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 269 amino acids
Theoretical weight: 29.69 KDa
Source organism: Methanothermobacter thermautotrophicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O26153 (Residues: 1-266; Coverage: 100%)
Gene name: MTH_47
Sequence domains: Amino acid kinase family
Structure domains: Acetylglutamate kinase-like

Ligands and Environments

2 bound ligands:
Ligand ADP 2 x ADP
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 119.672Å b: 96.36Å c: 72.43Å
α: 90° β: 120.59° γ: 90°
R-values:
R R work R free
0.208 0.205 0.259
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Enzymes of the mevalonate pathway of isoprenoid biosynthesis.
Miziorko HM. (2011)
4 more

3 mentions without citation

X-ray structures of isopentenyl phosphate kinase.
Mabanglo et al. (2010)
2 more