› 3l61

X-ray diffraction
1.5Å resolution

Crystal structure of substrate-free P450cam at 200 mM [K+]

Released:
DOI: 10.2210/pdb3l61/pdb
PDB entry 3l61 coloured by chain, front view.
PDB entry 3l61 coloured by chain, side view.
PDB entry 3l61 coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
P450cam visits an open conformation in the absence of substrate.
Lee YT, Wilson RF, Rupniewski I, Goodin DB
Biochemistry 49 3412-9 (2010)
PMID: 20297780

Function and Biology Details

Reaction catalysed: 
(+)-camphor + reduced putidaredoxin + O(2) = (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132314 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Camphor 5-monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 414 amino acids
Theoretical weight: 46.56 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00183 (Residues: 2-415; Coverage: 100%)
Gene names: camC, cyp101
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 65.606Å b: 73.825Å c: 92.428Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.197 0.216
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Heme enzyme structure and function.
Poulos TL. (2014)
6 more

15 mentions without citation

Recent Structural Insights into Cytochrome P450 Function.
Guengerich et al. (2016)
14 more