› 3l5w

X-ray diffraction
2Å resolution

Crystal structure of the complex between IL-13 and C836 FAB

Released:
DOI: 10.2210/pdb3l5w/pdb
PDB entry 3l5w coloured by chain, front view.
PDB entry 3l5w coloured by chain, side view.
PDB entry 3l5w coloured by chain, top view.
Source organisms:
Primary publication:
Human framework adaptation of a mouse anti-human IL-13 antibody.
Fransson J, Teplyakov A, Raghunathan G, Chi E, Cordier W, Dinh T, Feng Y, Giles-Komar J, Gilliland G, Lollo B, Malia TJ, Nishioka W, Obmolova G, Zhao S, Zhao Y, Swanson RV, Almagro JC
J Mol Biol 398 214-31 (2010)
PMID: 20226193

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211090 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
C836 LIGHT CHAIN Chains: A, L
Molecule details ›
Chains: A, L
Length: 214 amino acids
Theoretical weight: 23.5 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
C836 HEAVY CHAIN Chains: B, H
Molecule details ›
Chains: B, H
Length: 230 amino acids
Theoretical weight: 24.78 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
Interleukin-13 Chains: I, J
Molecule details ›
Chains: I, J
Length: 113 amino acids
Theoretical weight: 12.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35225 (Residues: 35-146; Coverage: 92%)
Gene names: IL13, NC30
Sequence domains: Interleukin-13
Structure domains: Growth Hormone; Chain: A;

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 76.62Å b: 65.56Å c: 118.74Å
α: 90° β: 107.02° γ: 90°
R-values:
R R work R free
0.208 0.205 0.253
Expression systems:
  • Cricetulus griseus
  • Escherichia coli

Quick links

Citations

2 review citations

Progress and Challenges in the Design and Clinical Development of Antibodies for Cancer Therapy.
Almagro et al. (2017)
1 more

11 mentions without citation

Updates to the Integrated Protein-Protein Interaction Benchmarks: Docking Benchmark Version 5 and Affinity Benchmark Version 2.
Vreven et al. (2015)
10 more