› 3l07

X-ray diffraction
1.88Å resolution

Methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase, putative bifunctional protein folD from Francisella tularensis.

Released:
DOI: 10.2210/pdb3l07/pdb
PDB entry 3l07 coloured by chain, front view.
PDB entry 3l07 coloured by chain, side view.
PDB entry 3l07 coloured by chain, top view.
Source organism: Francisella tularensis subsp. tularensis SCHU S4
Entry authors: Osipiuk J, Maltseva N, Mulligan R, Hasseman J, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reactions catalysed: 
5,10-methylenetetrahydrofolate + NADP(+) = 5,10-methenyltetrahydrofolate + NADPH
5,10-methenyltetrahydrofolate + H(2)O = 10-formyltetrahydrofolate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177688 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional protein FolD Chains: A, B
Molecule details ›
Chains: A, B
Length: 285 amino acids
Theoretical weight: 30.77 KDa
Source organism: Francisella tularensis subsp. tularensis SCHU S4
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5NGF3 (Residues: 1-282; Coverage: 100%)
Gene names: FTT_0892, folD
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand PO4 1 x PO4
Ligand IMD 2 x IMD
Ligand EDO 4 x EDO
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 70.299Å b: 73.385Å c: 106.916Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.165 0.208
Expression system: Escherichia coli

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