› 3ktv

X-ray diffraction
3.8Å resolution

Crystal structure of the human SRP19/S-domain SRP RNA complex

Released:
DOI: 10.2210/pdb3ktv/pdb
PDB entry 3ktv coloured by chain, front view.
PDB entry 3ktv coloured by chain, side view.
PDB entry 3ktv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into the assembly of the human and archaeal signal recognition particles.
Wild K, Bange G, Bozkurt G, Segnitz B, Hendricks A, Sinning I
Acta Crystallogr D Biol Crystallogr 66 295-303 (2010)
PMID: 20179341

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140558 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
Signal recognition particle 19 kDa protein Chains: B, D
Molecule details ›
Chains: B, D
Length: 128 amino acids
Theoretical weight: 14.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09132 (Residues: 1-120; Coverage: 83%)
Gene name: SRP19
Sequence domains: SRP19 protein
Structure domains: Signal recognition particle, SRP19-like subunit
SRP RNA Chain: A
Molecule details ›
Chain: A
Length: 108 nucleotides
Theoretical weight: 35.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Sequence domains: Metazoan signal recognition particle RNA
SRP RNA Chain: C
Molecule details ›
Chain: C
Length: 108 nucleotides
Theoretical weight: 35.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Sequence domains: Metazoan signal recognition particle RNA

Ligands and Environments

2 bound ligands:
Ligand K 3 x K
Ligand MG 3 x MG
1 modified residue:
Ligand GDP 1 x GDP

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P43212
Unit cell:
a: 100.12Å b: 100.12Å c: 293.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.293 0.291 0.329
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The Archaeal Signal Recognition Particle: Present Understanding and Future Perspective.
Gupta et al. (2017)
8 other articles

5 mentions without citation

Archaea signal recognition particle shows the way.
Zwieb et al. (2010)
4 more