Function and Biology Details
Reactions catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
Non-structural protein 5A and DNA (preferred)
PDBe Complex ID:
PDB-CPX-116498 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Serine protease/helicase NS3
Molecule details ›
Chains: A, B
Length: 436 amino acids
Theoretical weight: 46.67 KDa
Source organism: Hepatitis C virus (isolate Con1)
Expression system: Escherichia coli BL21(DE3)
UniProt:
Structure domains:
Length: 436 amino acids
Theoretical weight: 46.67 KDa
Source organism: Hepatitis C virus (isolate Con1)
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
Q9WMX2 (Residues: 1215-1650; Coverage: 15%)
Structure domains:
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
NSLS BEAMLINE X29A
Spacegroup:
P212121
Expression systems:
- Escherichia coli BL21(DE3)
- Not provided
